human sdc1 recombinant protein  (R&D Systems)

Journal: The Journal of surgical research

Article Title: Prolonged cardiopulmonary bypass is associated with endothelial glycocalyx degradation

doi: 10.1016/j.jss.2020.02.011

Figure Lengend Snippet: A-D. Levels of mtDNA (A, n=50), syndecan-1 (B, n=44), WBC (C, n=44), and IL-6 (D, n=44) were measured in blood plasma obtained from cardiac surgery patients before (pre-op), during surgery and at 4–8 hours, 24 hours and 96 hours (day 4) after surgery. Friedman test with Dunn’s multiple comparisons test. P values are indicated.

Article Snippet: Human recombinant syndecan-1 (11429-H08H, Sino Biologicals Wayne, PA) in phosphate buffered saline (PBS) was intravenously injected to FVB/NJ mice at a dose of 120 μg/kg body weight.

Techniques:

Journal: The Journal of surgical research

Article Title: Prolonged cardiopulmonary bypass is associated with endothelial glycocalyx degradation

doi: 10.1016/j.jss.2020.02.011

Figure Lengend Snippet: The correlation between CPB time and mtDNA (A, n=50), syndecan-1 (C, n=44), WBC (E-F, n=44), and IL-6 (G-H, n=44). The correlation analysis was performed at 4–8 hours (A, C, E and G) and 24 hours (F and H) after cardiac surgery. Spearman’s rank correlation coefficient and p values are indicated. The comparisons of mtDNA (B) and syndecan-1 (D) levels between groups of patients with shorter (grey-shaded circles) and longer (gray-shaded quadrants) CPB time. The statistical significance was calculated using repeated measures two-way ANOVA with Sidak’s multiple comparisons test. P values are indicated.

Article Snippet: Human recombinant syndecan-1 (11429-H08H, Sino Biologicals Wayne, PA) in phosphate buffered saline (PBS) was intravenously injected to FVB/NJ mice at a dose of 120 μg/kg body weight.

Techniques:

Journal: The Journal of surgical research

Article Title: Prolonged cardiopulmonary bypass is associated with endothelial glycocalyx degradation

doi: 10.1016/j.jss.2020.02.011

Figure Lengend Snippet: A. The correlation between level of soluble syndecan-1 and WBC at 4–8 hours after the surgery (n=44). B. Flow cytometry showing subpopulations of circulating WBC, stained with isotype-matched control (left) or antibody against CD45 (right). C. Number of neutrophils (left), monocytes (middle) and lymphocytes (right). Friedman test with Dunn’s multiple comparisons test. D. The relationships between levels of syndecan-1 and neutrophils (left), monocytes (middle), and lymphocytes (right). Spearman’s rank correlation coefficient and p values are indicated.

Article Snippet: Human recombinant syndecan-1 (11429-H08H, Sino Biologicals Wayne, PA) in phosphate buffered saline (PBS) was intravenously injected to FVB/NJ mice at a dose of 120 μg/kg body weight.

Techniques: Flow Cytometry, Staining

Journal: The Journal of surgical research

Article Title: Prolonged cardiopulmonary bypass is associated with endothelial glycocalyx degradation

doi: 10.1016/j.jss.2020.02.011

Figure Lengend Snippet: A. Flow cytometry plots showing percentage of CD11bposLy6Gpos circulating neutrophils (upper right quadrant), CD11bposLy6Gneg monocytes (upper left quadrant) and lymphocytes (lower left quadrant) 6 hours later after injection of PBS (control, left) or 120 μg/kg human recombinant syndecan-1 (right). B. Graphical representation of data from flow cytometry showing number of (left to right): WBC, neutrophils, monocytes, and lymphocytes after administration of PBS (n=6) or syndecan-1 (SDC1, n=7). Unpaired t test. C. Flow cytometry plots showing percentage of CD11bposLy6Gpos neutrophils (black gate) in bone marrow 6 hours later after injection of PBS (control, left) or 120 μg/kg human recombinant syndecan-1 (right). D. Number of CD45pos cells (left) and neutrophils (right) in murine bone marrow after administration of PBS (n=6) or syndecan-1 (SDC1, n=7). Unpaired t test.

Article Snippet: Human recombinant syndecan-1 (11429-H08H, Sino Biologicals Wayne, PA) in phosphate buffered saline (PBS) was intravenously injected to FVB/NJ mice at a dose of 120 μg/kg body weight.

Techniques: Flow Cytometry, Injection, Recombinant

Journal: The Journal of surgical research

Article Title: Prolonged cardiopulmonary bypass is associated with endothelial glycocalyx degradation

doi: 10.1016/j.jss.2020.02.011

Figure Lengend Snippet: A-D. Levels of mtDNA (A, n=50), syndecan-1 (B, n=44), WBC (C, n=44), and IL-6 (D, n=44) were measured in blood plasma obtained from cardiac surgery patients before (pre-op), during surgery and at 4–8 hours, 24 hours and 96 hours (day 4) after surgery. Friedman test with Dunn’s multiple comparisons test. P values are indicated.

Article Snippet: Administration of syndecan-1 Human recombinant syndecan-1 (11429-H08H, Sino Biologicals Wayne, PA) in phosphate buffered saline (PBS) was intravenously injected to FVB/NJ mice at a dose of 120 μg/kg body weight.

Techniques:

Journal: The Journal of surgical research

Article Title: Prolonged cardiopulmonary bypass is associated with endothelial glycocalyx degradation

doi: 10.1016/j.jss.2020.02.011

Figure Lengend Snippet: The correlation between CPB time and mtDNA (A, n=50), syndecan-1 (C, n=44), WBC (E-F, n=44), and IL-6 (G-H, n=44). The correlation analysis was performed at 4–8 hours (A, C, E and G) and 24 hours (F and H) after cardiac surgery. Spearman’s rank correlation coefficient and p values are indicated. The comparisons of mtDNA (B) and syndecan-1 (D) levels between groups of patients with shorter (grey-shaded circles) and longer (gray-shaded quadrants) CPB time. The statistical significance was calculated using repeated measures two-way ANOVA with Sidak’s multiple comparisons test. P values are indicated.

Article Snippet: Administration of syndecan-1 Human recombinant syndecan-1 (11429-H08H, Sino Biologicals Wayne, PA) in phosphate buffered saline (PBS) was intravenously injected to FVB/NJ mice at a dose of 120 μg/kg body weight.

Techniques:

Journal: The Journal of surgical research

Article Title: Prolonged cardiopulmonary bypass is associated with endothelial glycocalyx degradation

doi: 10.1016/j.jss.2020.02.011

Figure Lengend Snippet: A. The correlation between level of soluble syndecan-1 and WBC at 4–8 hours after the surgery (n=44). B. Flow cytometry showing subpopulations of circulating WBC, stained with isotype-matched control (left) or antibody against CD45 (right). C. Number of neutrophils (left), monocytes (middle) and lymphocytes (right). Friedman test with Dunn’s multiple comparisons test. D. The relationships between levels of syndecan-1 and neutrophils (left), monocytes (middle), and lymphocytes (right). Spearman’s rank correlation coefficient and p values are indicated.

Article Snippet: Administration of syndecan-1 Human recombinant syndecan-1 (11429-H08H, Sino Biologicals Wayne, PA) in phosphate buffered saline (PBS) was intravenously injected to FVB/NJ mice at a dose of 120 μg/kg body weight.

Techniques: Flow Cytometry, Staining

Journal: The Journal of surgical research

Article Title: Prolonged cardiopulmonary bypass is associated with endothelial glycocalyx degradation

doi: 10.1016/j.jss.2020.02.011

Figure Lengend Snippet: A. Flow cytometry plots showing percentage of CD11bposLy6Gpos circulating neutrophils (upper right quadrant), CD11bposLy6Gneg monocytes (upper left quadrant) and lymphocytes (lower left quadrant) 6 hours later after injection of PBS (control, left) or 120 μg/kg human recombinant syndecan-1 (right). B. Graphical representation of data from flow cytometry showing number of (left to right): WBC, neutrophils, monocytes, and lymphocytes after administration of PBS (n=6) or syndecan-1 (SDC1, n=7). Unpaired t test. C. Flow cytometry plots showing percentage of CD11bposLy6Gpos neutrophils (black gate) in bone marrow 6 hours later after injection of PBS (control, left) or 120 μg/kg human recombinant syndecan-1 (right). D. Number of CD45pos cells (left) and neutrophils (right) in murine bone marrow after administration of PBS (n=6) or syndecan-1 (SDC1, n=7). Unpaired t test.

Article Snippet: Administration of syndecan-1 Human recombinant syndecan-1 (11429-H08H, Sino Biologicals Wayne, PA) in phosphate buffered saline (PBS) was intravenously injected to FVB/NJ mice at a dose of 120 μg/kg body weight.

Techniques: Flow Cytometry, Injection, Recombinant